生物工程专业英语翻译,帮忙翻译一下吧.拜托啦、

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生物工程专业英语翻译,帮忙翻译一下吧.拜托啦、
2.8. RNase H assay
In a 10 Al volume, 1 nM a-32P-labeled uidA RNA
transcript, 50 nM oligodesoxynucleotide, 40 mM Tris–
HCl pH 7.5, 4 mM MgCl2, 1 mM DTT and 5 units
RNase H were mixed and incubated at 37 8C for 1 min.
The reaction was stopped by addition of 5 Al stopping
buffer. Reaction products were separated by polyacryl-
amide gel electrophoresis (5%) and gels were exposed
to an X-ray film after drying on Whatman filter paper.
Reaction products for each oligodesoxynucleotide were
determined twice in separate assays.
3. Results
3.1. In vitro analyses of ribozymes targeting the uidA
mRNA
The secondary structure of the uidA mRNA (1811 bp)
was analysed in silico using the program RNAdraw
(Matzura and Wennborg, 1996) and searched for sin-
gle-stranded regions containing a GUC sequence, which
follows the NHH rule and represents an efficient ribo-
zyme cleavage site (Ohkawa et al., 1995;

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