0.05mol/L磷酸盐缓冲液(pH7.0)如何配制?

题目

0.05mol/L磷酸盐缓冲液(pH7.0)如何配制?

答案

若pH偏高或偏低,可通过改变二者的比例来加以调整,室温保存即可.
pH 0.05mol/L NaH2PO4(ml) 0.05mol/L Na2HPO4(ml)
5.7 93.5 6.5
5.8 92.0 8.0
5.9 90.0 10.0
6.0 87.7 12.3
6.1 85.0 15.0
6.2 81.5 18.5
6.3 77.5 22.5
6.4 73.5 26.5
6.5 68.5 31.5
6.6 62.5 37.5
6.7 56.5 43.5
6.8 51.0 49.0
6.9 45.0 55.0
7.0 39.0 61.0
7.1 33.0 67.0
7.2 28.0 72.0
7.3 23.0 67.0
7.4 19.0 81.0
7.5 16.0 84.0
7.6 13.0 87.0
7.7 10.5 89.5
7.8 8.5 91.5
7.9 7.0 93.0
8.0 5.3 94.7
25℃下0.1mol/L磷酸钾缓冲液的配制
pH 0.05mol/L K2HPO4(ml) 0.05mol/L KH2PO4(ml)
5.8 8.5 91.5
6.0 13.2 86.8
6.2 19.2 80.8
6.4 27.8 72.2
6.6 38.1 61.9
6.8 49.7 50.3
7.0 61.5 38.5
7.2 71.7 28.3
7.4 80.2 19.8
7.6 86.6 13.4
7.8 90.8 9.2
8.0 94 6.0